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Identification of a proline-binding motif regulating CD2-triggered T lymphocyte activation.

ABSTRACT: An intracellular protein termed CD2 binding protein 2 (CD2BP2), which binds to a site containing two PPPGHR segments within the cytoplasmic region of CD2, was identified. Mutagenesis and NMR analysis demonstrated that the CD2 binding region of CD2BP2 includes a 17-aa motif (GPY[orF]xxxxM[orV]xxWxxx GYF), also found in several yeast and Caenorhabditis elegans proteins of unknown function. In Jurkat T cells, over-expression of the isolated CD2BP2 domain binding to CD2 enhances the production of interleukin 2 on crosslinking of CD2 but not the T cell receptor. Hence, a proline-binding module distinct from SH3 and WW domains regulates protein-protein interactions.

SUBMITTER: Nishizawa K

PROVIDER: S-EPMC24547 | biostudies-literature | 1998 Dec

REPOSITORIES: biostudies-literature

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Identification of a proline-binding motif regulating CD2-triggered T lymphocyte activation.

Nishizawa K K Freund C C Li J J Wagner G G Reinherz E L EL

Proceedings of the National Academy of Sciences of the United States of America 19981201 25

An intracellular protein termed CD2 binding protein 2 (CD2BP2), which binds to a site containing two PPPGHR segments within the cytoplasmic region of CD2, was identified. Mutagenesis and NMR analysis demonstrated that the CD2 binding region of CD2BP2 includes a 17-aa motif (GPY[orF]xxxxM[orV]xxWxxx GYF), also found in several yeast and Caenorhabditis elegans proteins of unknown function. In Jurkat T cells, over-expression of the isolated CD2BP2 domain binding to CD2 enhances the production of in ...[more]

PMID: 9843987

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