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The role of microtubule movement in bidirectional organelle transport.


ABSTRACT: We study the role of microtubule movement in bidirectional organelle transport in Drosophila S2 cells and show that EGFP-tagged peroxisomes in cells serve as sensitive probes of motor induced, noisy cytoskeletal motions. Multiple peroxisomes move in unison over large time windows and show correlations with microtubule tip positions, indicating rapid microtubule fluctuations in the longitudinal direction. We report the first high-resolution measurement of longitudinal microtubule fluctuations performed by tracing such pairs of co-moving peroxisomes. The resulting picture shows that motor-dependent longitudinal microtubule oscillations contribute significantly to cargo movement along microtubules. Thus, contrary to the conventional view, organelle transport cannot be described solely in terms of cargo movement along stationary microtubule tracks, but instead includes a strong contribution from the movement of the tracks.

SUBMITTER: Kulic IM 

PROVIDER: S-EPMC2481308 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature

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The role of microtubule movement in bidirectional organelle transport.

Kulic Igor M IM   Brown André E X AE   Kim Hwajin H   Kural Comert C   Blehm Benjamin B   Selvin Paul R PR   Nelson Philip C PC   Gelfand Vladimir I VI  

Proceedings of the National Academy of Sciences of the United States of America 20080714 29


We study the role of microtubule movement in bidirectional organelle transport in Drosophila S2 cells and show that EGFP-tagged peroxisomes in cells serve as sensitive probes of motor induced, noisy cytoskeletal motions. Multiple peroxisomes move in unison over large time windows and show correlations with microtubule tip positions, indicating rapid microtubule fluctuations in the longitudinal direction. We report the first high-resolution measurement of longitudinal microtubule fluctuations per  ...[more]

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2017-06-29 | GSE72664 | GEO