Ontology highlight
ABSTRACT:
SUBMITTER: Moonen MJ
PROVIDER: S-EPMC2493252 | biostudies-literature | 2008 Aug
REPOSITORIES: biostudies-literature
Moonen Mariëlle J H MJ Synowsky Silvia A SA van den Berg Willy A M WA Westphal Adrie H AH Heck Albert J R AJ van den Heuvel Robert H H RH Fraaije Marco W MW van Berkel Willem J H WJ
Journal of bacteriology 20080523 15
Hydroquinone 1,2-dioxygenase (HQDO), an enzyme involved in the catabolism of 4-hydroxyacetophenone in Pseudomonas fluorescens ACB, was purified to apparent homogeneity. Ligandation with 4-hydroxybenzoate prevented the enzyme from irreversible inactivation. HQDO was activated by iron(II) ions and catalyzed the ring fission of a wide range of hydroquinones to the corresponding 4-hydroxymuconic semialdehydes. HQDO was inactivated by 2,2'-dipyridyl, o-phenanthroline, and hydrogen peroxide and inhibi ...[more]