Ontology highlight
ABSTRACT:
SUBMITTER: Zhou HX
PROVIDER: S-EPMC24950 | biostudies-literature | 1997 Nov
REPOSITORIES: biostudies-literature
Zhou H X HX Wong K Y KY Vijayakumar M M
Proceedings of the National Academy of Sciences of the United States of America 19971101 23
The diffusional encounter between substrate and enzyme, and hence catalytic efficiency, can be enhanced by mutating charged residues on the surface of the enzyme. In this paper we present a simple method for screening such mutations. This is based on our earlier result that electrostatic enhancement of the enzyme-substrate binding rate constant can be accounted for just by the interaction potential within the active site. Assuming that catalytic and structural integrity is maintained, the cataly ...[more]