Structure of Deinococcus radiodurans tunicamycin-resistance protein (TmrD), a phosphotransferase.
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SUBMITTER: Kapp U
PROVIDER: S-EPMC2496873 | biostudies-literature | 2008 Jun
REPOSITORIES: biostudies-literature
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Structure of Deinococcus radiodurans tunicamycin-resistance protein (TmrD), a phosphotransferase.
Kapp Ulrike U Macedo Sofia S Hall David Richard DR Leiros Ingar I McSweeney Sean M SM Mitchell Edward E
Acta crystallographica. Section F, Structural biology and crystallization communications 20080516 Pt 6
The open-reading frame (ORF) DR_1419 in the Deinococcus radiodurans genome is annotated as a representative of the wide family of tunicamycin-resistance proteins as identified in a range of bacterial genomes. The D. radiodurans ORF DR_1419 was cloned and expressed; the protein TmrD was crystallized and its X-ray crystal structure was determined to 1.95 A resolution. The structure was determined using single-wavelength anomalous diffraction with selenomethionine-derivatized protein. The refined s ...[more]
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