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The CHD3 remodeler PICKLE promotes trimethylation of histone H3 lysine 27.


ABSTRACT: CHD3 proteins are ATP-dependent chromatin remodelers that contribute to repression of developmentally regulated genes in both animal and plant systems. In animals, this repression has been linked to a multiple subunit complex, Mi-2/NuRD, whose constituents include a CHD3 protein, a histone deacetylase, and a methyl-CpG-binding domain protein. In Arabidopsis, PICKLE (PKL) codes for a CHD3 protein that acts during germination to repress expression of seed-associated genes. Repression of seed-associated traits is promoted in pkl seedlings by the plant growth regulator gibberellin (GA). We undertook a microarray analysis to determine how PKL and GA act to promote the transition from seed to seedling. We found that PKL and GA act in separate pathways to repress expression of seed-specific genes. Comparison of genomic datasets revealed that PKL-dependent genes are enriched for trimethylation of histone H3 lysine 27 (H3K27me3), a repressive epigenetic mark. Chromatin immunoprecipitation studies demonstrate that PKL promotes H3K27me3 in both germinating seedlings and in adult plants but do not identify a connection between PKL-dependent expression and acetylation levels. Taken together, our analyses illuminate a new pathway by which CHD3 remodelers contribute to repression in eukaryotes.

SUBMITTER: Zhang H 

PROVIDER: S-EPMC2504882 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature

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The CHD3 remodeler PICKLE promotes trimethylation of histone H3 lysine 27.

Zhang Heng H   Rider Stanley Dean SD   Henderson James T JT   Fountain Matthew M   Chuang King K   Kandachar Vasundhara V   Simons Alexis A   Edenberg Howard J HJ   Romero-Severson Jeanne J   Muir William M WM   Ogas Joe J  

The Journal of biological chemistry 20080606 33


CHD3 proteins are ATP-dependent chromatin remodelers that contribute to repression of developmentally regulated genes in both animal and plant systems. In animals, this repression has been linked to a multiple subunit complex, Mi-2/NuRD, whose constituents include a CHD3 protein, a histone deacetylase, and a methyl-CpG-binding domain protein. In Arabidopsis, PICKLE (PKL) codes for a CHD3 protein that acts during germination to repress expression of seed-associated genes. Repression of seed-assoc  ...[more]

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