Metabolic and signaling properties of an Itpk gene family in Glycine max.
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ABSTRACT:
SUBMITTER: Stiles AR
PROVIDER: S-EPMC2515385 | biostudies-literature | 2008 Jun
REPOSITORIES: biostudies-literature
Publications
Metabolic and signaling properties of an Itpk gene family in Glycine max.
Stiles Amanda R AR Qian Xun X Shears Stephen B SB Grabau Elizabeth A EA
FEBS letters 20080512 13
We have cloned and characterized four Itpk genes from soybean. All four recombinant Itpk proteins showed canonical Ins(1,3,4)P3 5/6-kinase activity, but a kinetic analysis raised questions about its biological significance. Instead, we provide evidence that one alternative biological role for soybean Itpks is to interconvert the Cl(-) channel inhibitor, Ins(3,4,5,6)P4, and its metabolic precursor, Ins(1,3,4,5,6)P5, within a substrate cycle. The soybean Itpks also phosphorylated Ins(3,4,6)P3 to I ...[more]
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