Ontology highlight
ABSTRACT:
SUBMITTER: Fafarman AT
PROVIDER: S-EPMC2516909 | biostudies-literature | 2006 Oct
REPOSITORIES: biostudies-literature
Fafarman Aaron T AT Webb Lauren J LJ Chuang Jessica I JI Boxer Steven G SG
Journal of the American Chemical Society 20061001 41
The nitrile stretching mode of the thiocyanate moiety is a nearly ideal probe for measuring the local electric field arising from the organized environment of the interior of a protein. Nitriles were introduced into three proteins: ribonuclease S (RNase S), human aldose reductase (hALR2), and the reaction center (RC) of Rhodobacter capsulatus, through a facile synthetic scheme for the transformation of cysteine residues into thiocyanatoalanine. Vibrational Stark effect spectroscopy and Fourier t ...[more]