Ontology highlight
ABSTRACT:
SUBMITTER: Yakamavich JA
PROVIDER: S-EPMC2517146 | biostudies-literature | 2008 Jul
REPOSITORIES: biostudies-literature
Yakamavich Joseph A JA Baker Tania A TA Sauer Robert T RT
Journal of molecular biology 20080604 5
ATP binding and hydrolysis are critical for protein degradation by HslUV, a AAA(+) machine containing one or two HslU(6) ATPases and the HslV(12) peptidase. Although each HslU homohexamer has six potential ATP-binding sites, we show that only three or four ATP molecules bind at saturation and present evidence for three functional subunit classes. These results imply that only a subset of HslU and HslUV crystal structures represents functional enzyme conformations. Our results support an asymmetr ...[more]