Protease-catalysed direct asymmetric Mannich reaction in organic solvent.

ABSTRACT: We reported the first enzyme-catalysed, direct, three-component asymmetric Mannich reaction using protease type XIV from Streptomyces griseus (SGP) in acetonitrile. Yields of up to 92% with enantioselectivities of up to 88% e.e. and diastereoselectivities of up to 92:8 (syn:anti) were achieved under the optimised conditions. This enzyme's catalytic promiscuity expands the application of this biocatalyst and provides a potential alternative method for asymmetric Mannich reactions.

SUBMITTER: Xue Y 

PROVIDER: S-EPMC3478581 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature