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Synthesis, characterization, and utility of thermoresponsive natural/unnatural product macroligands for affinity chromatography.

ABSTRACT: [Structure: see text] The synthesis and characterization of thermoresponsive, water-soluble poly-N-isopropyl acrylamide (PNIPAM) derived macroligands displaying cyclosporin A (CsA) and dexamethasone (Dex) for use as novel affinity resins are described. Characterization of these soluble macroligands, including ligand loading and integrity, was determined by 1H NMR spectroscopy. One of the CsA macroligands was used in a protein affinity experiment to capture known binding proteins of CsA, the cyclophilins, from Jurkat T-cell lysates.

SUBMITTER: Zhou M

PROVIDER: S-EPMC2517857 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature

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Synthesis, characterization, and utility of thermoresponsive natural/unnatural product macroligands for affinity chromatography.

Zhou Min M Sivaramakrishnan Ananthapadmanab A Ponnamperuma Krishan K Low Woon-Kai WK Li Chunmei C Liu Jun O JO Bergbreiter David E DE Romo Daniel D

Organic letters 20061101 23

[Structure: see text] The synthesis and characterization of thermoresponsive, water-soluble poly-N-isopropyl acrylamide (PNIPAM) derived macroligands displaying cyclosporin A (CsA) and dexamethasone (Dex) for use as novel affinity resins are described. Characterization of these soluble macroligands, including ligand loading and integrity, was determined by 1H NMR spectroscopy. One of the CsA macroligands was used in a protein affinity experiment to capture known binding proteins of CsA, the cycl ...[more]

PMID: 17078689

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