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Participation of the tRNA A76 hydroxyl groups throughout translation.

ABSTRACT: The free 2'-3' cis-diol at the 3'-terminus of tRNA provides a unique juxtaposition of functional groups that play critical roles during protein synthesis. The translation process involves universally conserved chemistry at almost every stage of this multistep procedure, and the 2'- and 3'-OHs are in the immediate vicinity of chemistry at each step. The cis-diol contribution affects steps ranging from tRNA aminoacylation to peptide bond formation. The contributions have been studied in assays related to translation over a period that spans at least three decades. In this review, we follow the 2'- and 3'-OHs through the steps of translation and examine the involvement of these critical functional groups.

SUBMITTER: Weinger JS

PROVIDER: S-EPMC2522371 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature

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Participation of the tRNA A76 hydroxyl groups throughout translation.

Weinger Joshua S JS Strobel Scott A SA

Biochemistry 20060501 19

The free 2'-3' cis-diol at the 3'-terminus of tRNA provides a unique juxtaposition of functional groups that play critical roles during protein synthesis. The translation process involves universally conserved chemistry at almost every stage of this multistep procedure, and the 2'- and 3'-OHs are in the immediate vicinity of chemistry at each step. The cis-diol contribution affects steps ranging from tRNA aminoacylation to peptide bond formation. The contributions have been studied in assays rel ...[more]

PMID: 16681365

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