Unknown

Dataset Information

0

Endometrial-peritoneal interactions during endometriotic lesion establishment.


ABSTRACT: The pathophysiology of endometriosis remains unclear but involves a complex interaction between ectopic endometrium and host peritoneal tissues. We hypothesized that disruption of this interaction would suppress endometriotic lesion formation. We hoped to delineate the molecular and cellular dialogue between ectopic human endometrium and peritoneal tissues in nude mice as a first step toward testing this hypothesis. Human endometrium was xenografted into nude mice, and the resulting lesions were analyzed using microarrays. A novel technique was developed that unambiguously determined whether RNA transcripts identified via microarray analyses originated from human cells (endometrium) or mouse cells (mesothelium). Four key pathways (ubiquitin/proteasome, inflammation, tissue remodeling/repair, and ras-mediated oncogenesis) were revealed, demonstrating communication between host mesothelial cells and ectopic endometrium. Morphometric analysis of nude mouse lesions confirmed that necrosis, inflammation, healing and repair, and cell proliferation occurred during xenograft development. These processes were entirely consistent with the molecular networks revealed by the microarray data. The transcripts detected in the xenografts overlapped with differentially expressed transcripts in a comparison between paired eutopic and ectopic endometria from human endometriotic patients. For the first time, components of the interaction between ectopic endometrium and peritoneal stromal tissues are revealed. Targeted disruption of this dialogue is likely to inhibit endometriotic tissue formation and may prove to be an effective therapeutic strategy for endometriosis.

SUBMITTER: Hull ML 

PROVIDER: S-EPMC2527068 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Endometrial-peritoneal interactions during endometriotic lesion establishment.

Hull M Louise ML   Escareno Claudia Rangel CR   Godsland Jane M JM   Doig John R JR   Johnson Claire M CM   Phillips Stephen C SC   Smith Stephen K SK   Tavaré Simon S   Print Cristin G CG   Charnock-Jones D Stephen DS  

The American journal of pathology 20080807 3


The pathophysiology of endometriosis remains unclear but involves a complex interaction between ectopic endometrium and host peritoneal tissues. We hypothesized that disruption of this interaction would suppress endometriotic lesion formation. We hoped to delineate the molecular and cellular dialogue between ectopic human endometrium and peritoneal tissues in nude mice as a first step toward testing this hypothesis. Human endometrium was xenografted into nude mice, and the resulting lesions were  ...[more]

Similar Datasets

2015-01-06 | GSE56414 | GEO
2022-06-15 | GSE202661 | GEO
| S-EPMC8613489 | biostudies-literature
| S-EPMC4061076 | biostudies-literature
2024-04-16 | GSE263897 | GEO
| S-EPMC7529860 | biostudies-literature
| S-EPMC2727462 | biostudies-literature
2014-08-01 | E-GEOD-58178 | biostudies-arrayexpress
| PRJNA836898 | ENA
| S-EPMC4227690 | biostudies-literature