Ontology highlight
ABSTRACT:
SUBMITTER: Subramanian VS
PROVIDER: S-EPMC2528843 | biostudies-literature | 2008 Sep
REPOSITORIES: biostudies-literature
Subramanian Veedamali S VS Marchant Jonathan S JS Reidling Jack C JC Said Hamid M HM
Biochemical and biophysical research communications 20080709 1
The human sodium-dependent vitamin C transporters (hSVCT1 and hSVCT2) mediate cellular uptake of ascorbic acid. Both these transporters contain potential sites for N-glycosylation in their extracellular domains (Asn-138, Asn-144 [hSVCT1]; Asn-188, Asn-196 [hSVCT2]), however the role of N-glycosylation in transporter function is unexplored. On the basis of the result that tunicamycin decreased (14)C-ascorbic acid uptake in HepG2 cells, we systematically ablated all consensus N-glycosylation sites ...[more]