Unknown

Dataset Information

0

PCSK9 is required for the disposal of non-acetylated intermediates of the nascent membrane protein BACE1.


ABSTRACT: We have recently identified a new form of post-translational regulation of BACE1 (beta-site amyloid precursor protein (APP)-cleaving enzyme 1), a membrane protein that acts as the rate-limiting enzyme in the generation of the Alzheimer disease amyloid beta-peptide (Abeta). Specifically, BACE1 is transiently acetylated on seven lysine residues in the lumen of the endoplasmic reticulum/endoplasmic reticulum-Golgi intermediate compartment (ER/ERGIC). The acetylated intermediates of the nascent protein are able to reach the Golgi apparatus, whereas the non-acetylated ones are retained and degraded in a post-ER compartment. Here, we report that the serine protease PCSK9 (proprotein convertase subtilisin kexin type 9) contributes to the disposal of non-acetylated BACE1. Both overexpression and small interfering RNA-mediated downregulation of PCSK9 affected the levels of BACE1. The downregulation of PCSK9 affected the levels of the loss-of-acetylation mutants (BACE1(Ala) and BACE1(Arg)) but not those of the gain-of-acetylation mutant (BACE1(Gln)). In addition, Pcsk9(-/-) mice showed increased levels of BACE1 and Abeta in the brain. Finally, we found that nascent low-density lipoprotein receptor, a known substrate of PCSK9, is also acetylated.

SUBMITTER: Jonas MC 

PROVIDER: S-EPMC2529349 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

PCSK9 is required for the disposal of non-acetylated intermediates of the nascent membrane protein BACE1.

Jonas Mary Cabell MC   Costantini Claudio C   Puglielli Luigi L  

EMBO reports 20080725 9


We have recently identified a new form of post-translational regulation of BACE1 (beta-site amyloid precursor protein (APP)-cleaving enzyme 1), a membrane protein that acts as the rate-limiting enzyme in the generation of the Alzheimer disease amyloid beta-peptide (Abeta). Specifically, BACE1 is transiently acetylated on seven lysine residues in the lumen of the endoplasmic reticulum/endoplasmic reticulum-Golgi intermediate compartment (ER/ERGIC). The acetylated intermediates of the nascent prot  ...[more]

Similar Datasets

| S-EPMC7202230 | biostudies-literature
| S-EPMC7613651 | biostudies-literature
| S-SCDT-EMBOR-2019-48835V1 | biostudies-other
| S-EPMC6640710 | biostudies-literature
| S-EPMC2782093 | biostudies-literature
| S-EPMC20294 | biostudies-literature
| S-EPMC4215243 | biostudies-literature
| S-EPMC7367713 | biostudies-literature
| S-EPMC8576036 | biostudies-literature
| S-EPMC9303681 | biostudies-literature