SGTA associates with nascent membrane protein precursors

ABSTRACT: The endoplasmic reticulum (ER) is a major site for membrane protein synthesis in eukaryotes. The majority of integral membrane proteins are delivered to the ER membrane via the co-translational, signal recognition particle (SRP) dependent, route. However, tail-anchored proteins employ an alternative, post-translational, route(s) that relies on distinct factors such as a cytosolic protein quality control component, SGTA. We now show that SGTA is selectively recruited to ribosomes synthesizing a diverse range of membrane proteins, suggesting that its biosynthetic client base also includes precursors on the co-translational ER delivery pathway. Strikingly, SGTA is recruited to nascent membrane proteins before their transmembrane domain emerges from the ribosome. Hence, SGTA is ideally placed

SUBMITTER: Dr. Pawel Leznicki 

PROVIDER: S-SCDT-EMBOR-2019-48835V1 | biostudies-other |

REPOSITORIES: biostudies-other