L-Enantiomers of transition state analogue inhibitors bound to human purine nucleoside phosphorylase.
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ABSTRACT:
SUBMITTER: Rinaldo-Matthis A
PROVIDER: S-EPMC2531256 | biostudies-literature | 2008 Jan
REPOSITORIES: biostudies-literature
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L-Enantiomers of transition state analogue inhibitors bound to human purine nucleoside phosphorylase.
Rinaldo-Matthis Agnes A Murkin Andrew S AS Ramagopal Udupi A UA Clinch Keith K Mee Simon P H SP Evans Gary B GB Tyler Peter C PC Furneaux Richard H RH Almo Steven C SC Schramm Vern L VL
Journal of the American Chemical Society 20071223 3
Human purine nucleoside phosphorylase (PNP) was crystallized with transition-state analogue inhibitors Immucillin-H and DADMe-Immucillin-H synthesized with ribosyl mimics of l-stereochemistry. The inhibitors demonstrate that major driving forces for tight binding of these analogues are the leaving group interaction and the cationic mimicry of the transition state, even though large geometric changes occur with d-Immucillins and l-Immucillins bound to human PNP. ...[more]
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