Ontology highlight
ABSTRACT:
SUBMITTER: Quaderer R
PROVIDER: S-EPMC2533730 | biostudies-literature | 2006 Oct
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20061001 40
A gene cluster encoding all of the enzymes for the biosynthesis of the antibiotic pentalenolactone (1) has recently been identified in Streptomyces avermitilis. The biosynthetic gene cluster contains the ptlI (SAV2999) gene which encodes a cytochrome P450 (CYP183A1). PtlI was cloned by PCR and expressed in Escherichia coli as a C-terminal His6-tag protein. Recombinant PtlI bound pentalenene (3) with high affinity (KD = 1.44 +/- 0.14 muM). Incubation of recombinant PtlI with (+/-)-3 in the presen ...[more]