De novo design of a single-chain diphenylporphyrin metalloprotein.
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ABSTRACT:
SUBMITTER: Bender GM
PROVIDER: S-EPMC2542652 | biostudies-literature | 2007 Sep
REPOSITORIES: biostudies-literature
Publications
De novo design of a single-chain diphenylporphyrin metalloprotein.
Bender Gretchen M GM Lehmann Andreas A Zou Hongling H Cheng Hong H Fry H Christopher HC Engel Don D Therien Michael J MJ Blasie J Kent JK Roder Heinrich H Saven Jeffrey G JG DeGrado William F WF
Journal of the American Chemical Society 20070810 35
We describe the computational design of a single-chain four-helix bundle that noncovalently self-assembles with fully synthetic non-natural porphyrin cofactors. With this strategy, both the electronic structure of the cofactor as well as its protein environment may be varied to explore and modulate the functional and photophysical properties of the assembly. Solution characterization (NMR, UV-vis) of the protein showed that it bound with high specificity to the desired cofactors, suggesting that ...[more]
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