De novo enzyme design using Rosetta3.
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ABSTRACT:
SUBMITTER: Richter F
PROVIDER: S-EPMC3095599 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Publications
De novo enzyme design using Rosetta3.
Richter Florian F Leaver-Fay Andrew A Khare Sagar D SD Bjelic Sinisa S Baker David D
PloS one 20110516 5
The Rosetta de novo enzyme design protocol has been used to design enzyme catalysts for a variety of chemical reactions, and in principle can be applied to any arbitrary chemical reaction of interest. The process has four stages: 1) choice of a catalytic mechanism and corresponding minimal model active site, 2) identification of sites in a set of scaffold proteins where this minimal active site can be realized, 3) optimization of the identities of the surrounding residues for stabilizing interac ...[more]
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