Ontology highlight
ABSTRACT:
SUBMITTER: Kurschus FC
PROVIDER: S-EPMC2544534 | biostudies-literature | 2008 Sep
REPOSITORIES: biostudies-literature
Kurschus Florian C FC Fellows Edward E Stegmann Elisabeth E Jenne Dieter E DE
Proceedings of the National Academy of Sciences of the United States of America 20080904 37
How granzymes gain entry into the cytosol of target cells during killer cell attack has been the subject of several studies in the past, but the effective delivery mechanism during target cell encounter has not been clarified. Here we show that granzyme B (GzmB) mutants lacking binding to negatively charged, essentially heparan-sulfate-containing membrane receptors are poorly endocytosed yet are delivered to the cytosol with efficacy similar to that of WT GzmB. In a cell-based system GzmB-defici ...[more]