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Antibody interference with N-acyl homoserine lactone-mediated bacterial quorum sensing.

ABSTRACT: Many bacterial pathogens coordinate their virulence factor expression in a cell density-dependent manner. This population-dependent coordination of gene expression in bacteria has been termed "quorum sensing" (QS). N-Acyl homoserine lactones (AHLs) are used by over 70 Gram-negative bacterial species as autoinducers. Inhibition of QS signaling might represent a new target for antimicrobial therapy. Here we report the hapten design, synthesis, generation of monoclonal antibodies (mAbs) against AHLs, and the evaluation of these mAbs for their ability to blunt QS signaling and inhibit virulence factor expression in P. aeruginosa. The mAbs can be envisioned as a tool for future investigations into AHL-based QS, which may aid in gaining new insights into the pathogenesis of P. aeruginosa and may ultimately lead to the development of new strategies to combat bacterial diseases.

SUBMITTER: Kaufmann GF 

PROVIDER: S-EPMC2546487 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature

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Antibody interference with N-acyl homoserine lactone-mediated bacterial quorum sensing.

Kaufmann Gunnar F GF   Sartorio Rafaella R   Lee Sang-Hyeup SH   Mee Jenny M JM   Altobell Laurence J LJ   Kujawa David P DP   Jeffries Emily E   Clapham Bruce B   Meijler Michael M MM   Janda Kim D KD  

Journal of the American Chemical Society 20060301 9

Many bacterial pathogens coordinate their virulence factor expression in a cell density-dependent manner. This population-dependent coordination of gene expression in bacteria has been termed "quorum sensing" (QS). N-Acyl homoserine lactones (AHLs) are used by over 70 Gram-negative bacterial species as autoinducers. Inhibition of QS signaling might represent a new target for antimicrobial therapy. Here we report the hapten design, synthesis, generation of monoclonal antibodies (mAbs) against AHL  ...[more]

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