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Impact of phenolic compounds in the acyl homoserine lactone-mediated quorum sensing regulatory pathways.


ABSTRACT: Quorum sensing (QS) is a cell density-dependent regulation of virulent bacterial gene expression by autoinducers that potentially pertains in the epidemic of bacterial virulence. This study was initially designed to evaluate the effect of 5 phenolic compounds in the modulation of QS and virulence factors of Chromobacterium violaceum and Pseudomonas aeruginosa, and to determine the mechanisms of their effects. Biosensor strains were used to assess antibacterial and anti-QS effect of these compounds. Only methyl gallate (MG) among these compounds demonstrated profound anti-QS effect in the preliminary study, and thus only MG was utilized further to evaluate the effects on the synthesis and activity of acyl homoserine lactone (AHL) in C. violaceum and on the modulation of biofilm, motility, proteolytic, elastase, pyocyanin, and rhamnolipid activity in P. aeruginosa. Finally, the effect of MG on the expression of QS-regulated genes of P. aeruginosa was verified. MG suppressed both the synthesis and activity of AHL in C. violaceum. It also restricted the biofilm formation and other QS-associated virulence factor of P. aeruginosa. MG concentration-dependently suppressed the expression of lasI/R, rhlI/R, and pqsA of P. aeruginosa and was non-toxic in in vitro study. This is the first report of the anti-QS mechanism of MG.

SUBMITTER: Hossain MA 

PROVIDER: S-EPMC5587592 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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Impact of phenolic compounds in the acyl homoserine lactone-mediated quorum sensing regulatory pathways.

Hossain Md Akil MA   Lee Seung-Jin SJ   Park Na-Hye NH   Mechesso Abraham Fikru AF   Birhanu Biruk Tesfaye BT   Kang JeongWoo J   Reza Md Ahsanur MA   Suh Joo-Won JW   Park Seung-Chun SC  

Scientific reports 20170906 1


Quorum sensing (QS) is a cell density-dependent regulation of virulent bacterial gene expression by autoinducers that potentially pertains in the epidemic of bacterial virulence. This study was initially designed to evaluate the effect of 5 phenolic compounds in the modulation of QS and virulence factors of Chromobacterium violaceum and Pseudomonas aeruginosa, and to determine the mechanisms of their effects. Biosensor strains were used to assess antibacterial and anti-QS effect of these compoun  ...[more]

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