Dataset Information
YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules.
Ontology highlight
ABSTRACT: Background
YB-1 is a major regulator of gene expression in eukaryotic cells. In addition to its role in transcription, YB-1 plays a key role in translation and stabilization of mRNAs.Results
We show here that YB-1 interacts with tubulin and microtubules and stimulates microtubule assembly in vitro. High resolution imaging via electron and atomic force microscopy revealed that microtubules assembled in the presence of YB-1 exhibited a normal single wall ultrastructure and indicated that YB-1 most probably coats the outer microtubule wall. Furthermore, we found that YB-1 also promotes the assembly of MAPs-tubulin and subtilisin-treated tubulin. Finally, we demonstrated that tubulin interferes with RNA:YB-1 complexes.Conclusion
These results suggest that YB-1 may regulate microtubule assembly in vivo and that its interaction with tubulin may contribute to the control of mRNA translation.
SUBMITTER: Chernov KG
PROVIDER: S-EPMC2557009 | biostudies-literature | 2008 Sep
REPOSITORIES: biostudies-literature
Publications
YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules.
BMC biochemistry 20080915
<h4>Background</h4>YB-1 is a major regulator of gene expression in eukaryotic cells. In addition to its role in transcription, YB-1 plays a key role in translation and stabilization of mRNAs.<h4>Results</h4>We show here that YB-1 interacts with tubulin and microtubules and stimulates microtubule assembly in vitro. High resolution imaging via electron and atomic force microscopy revealed that microtubules assembled in the presence of YB-1 exhibited a normal single wall ultrastructure and indicate ...[more]