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Pathways change in expression during replicative aging in Saccharomyces cerevisiae.


ABSTRACT: Yeast replicative aging is a process resembling replicative aging in mammalian cells. During aging, wild-type haploid yeast cells enlarge, become sterile, and undergo nucleolar enlargement and fragmentation; we sought gene expression changes during the time of these phenotypic changes. Gene expression studied via microarrays and quantitative real-time reverse-transcription polymerase chain reaction (qPCR) has shown reproducible, statistically significant changes in messenger RNA (mRNA) of genes at 12 and 18-20 generations. Our findings support previously described changes towards aerobic metabolism, decreased ribosome gene expression, and a partial environmental stress response. Our findings include a pseudostationary phase, downregulation of methylation-related metabolism, increased nucleotide excision repair-related mRNA, and a strong upregulation of many of the regulatory subunits of protein phosphatase I (Glc7). These findings are correlated with aging changes in higher organisms as well as with the known involvement of protein phosphorylation states during yeast aging.

SUBMITTER: Yiu G 

PROVIDER: S-EPMC2562229 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature

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Pathways change in expression during replicative aging in Saccharomyces cerevisiae.

Yiu Gloria G   McCord Alejandra A   Wise Alison A   Jindal Rishi R   Hardee Jennifer J   Kuo Allen A   Shimogawa Michelle Yuen MY   Cahoon Laty L   Wu Michelle M   Kloke John J   Hardin Johanna J   Mays Hoopes Laura L LL  

The journals of gerontology. Series A, Biological sciences and medical sciences 20080101 1


Yeast replicative aging is a process resembling replicative aging in mammalian cells. During aging, wild-type haploid yeast cells enlarge, become sterile, and undergo nucleolar enlargement and fragmentation; we sought gene expression changes during the time of these phenotypic changes. Gene expression studied via microarrays and quantitative real-time reverse-transcription polymerase chain reaction (qPCR) has shown reproducible, statistically significant changes in messenger RNA (mRNA) of genes  ...[more]

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