Integration of 18O labeling and solution isoelectric focusing in a shotgun analysis of mitochondrial proteins.
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ABSTRACT:
SUBMITTER: Wang J
PROVIDER: S-EPMC2562304 | biostudies-literature | 2007 Dec
REPOSITORIES: biostudies-literature
Publications
Integration of 18O labeling and solution isoelectric focusing in a shotgun analysis of mitochondrial proteins.
Wang Jinshan J Gutierrez Peter P Edwards Nathan N Fenselau Catherine C
Journal of proteome research 20071110 12
Forward and reverse (18)O labeling are integrated with solution isoelectric focusing and capillary LC-tandem mass spectrometry to evaluate a new strategy for quantitative proteomics and to study abundance changes in mitochondrial proteins associated with drug resistance in MCF-7 human cancer cells. Galectin-3 binding protein, which is involved in apoptosis, was detected only in the resistant cell line, as a result of reverse labeling. Among 278 proteins identified, 12 were detected with abundanc ...[more]
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