Unknown

Dataset Information

0

Characterization of a recombinant Newcastle disease virus vaccine strain.


ABSTRACT: A recombinant La Sota strain (KBNP-C4152R2L) in which fusion (F) and hemagglutinin-neuraminidase (HN) genes were replaced with those of a contemporary genotype VIId virus, KBNP-4152, has been developed. To attenuate the virulence of the recombinant strain, the F cleavage motif was mutated from (112)RRQKR(116) to (112)GRQAR(116), and to reduce pathogenic instability, a codon which does not allow changes to basic amino acids by single point mutation was inserted at codon 115. In addition a six-nucleotide sequence was inserted into the intergenic region between matrix protein and F genes for attenuation without breaking the "rule-of-six." The HN protein length was increased from 571 to 577 as a marker. Serological tests revealed that the antigenicity of KBNP-C4152R2L was similar to that of KBNP-4152 but distinct from that of the La Sota strain. KBNP-C4152R2L was avirulent (intracerebral pathogenicity index, 0.0; mean death time, >168 h) and stable in pathogenicity through in vivo passages. The killed oil emulsion of and live KBNP-C4152R2L were completely protective against mortality and egg drop caused by virulent strains, and KBNP-C4152R2L was applicable to in ovo vaccination. Therefore, KBNP-C4152R2L is a promising vaccine strain and viral vector in terms of antigenicity, productivity, safety, and pathogenic stability.

SUBMITTER: Cho SH 

PROVIDER: S-EPMC2565930 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Characterization of a recombinant Newcastle disease virus vaccine strain.

Cho Sun-Hee SH   Kwon Hyuk-Joon HJ   Kim Tae-Eun TE   Kim Jae-Hong JH   Yoo Han-Sang HS   Park Man-Hoon MH   Park Young-Ho YH   Kim Sun-Joong SJ  

Clinical and vaccine immunology : CVI 20080903 10


A recombinant La Sota strain (KBNP-C4152R2L) in which fusion (F) and hemagglutinin-neuraminidase (HN) genes were replaced with those of a contemporary genotype VIId virus, KBNP-4152, has been developed. To attenuate the virulence of the recombinant strain, the F cleavage motif was mutated from (112)RRQKR(116) to (112)GRQAR(116), and to reduce pathogenic instability, a codon which does not allow changes to basic amino acids by single point mutation was inserted at codon 115. In addition a six-nuc  ...[more]

Similar Datasets

| S-EPMC114773 | biostudies-literature
| S-EPMC2878970 | biostudies-other
| S-EPMC3503142 | biostudies-literature
| S-EPMC5306239 | biostudies-literature
| S-EPMC4045777 | biostudies-literature
| S-EPMC5772752 | biostudies-literature
| S-EPMC6371441 | biostudies-literature
| S-EPMC7597455 | biostudies-literature
| S-EPMC6245631 | biostudies-literature
| S-EPMC6789757 | biostudies-literature