Ontology highlight
ABSTRACT:
SUBMITTER: Zhang L
PROVIDER: S-EPMC2565996 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
Zhang Lin L Ou Xijun X Zhao Guoping G Ding Xiaoming X
Journal of bacteriology 20080808 19
The Streptomyces phage phiBT1 encodes a site-specific integrase of the large serine recombinase subfamily. In this report, the enzymatic activity of the phiBT1 integrase was characterized in vitro. We showed that this integrase has efficient integration activity with substrate DNAs containing attB and attP sites, independent of DNA supercoiling or cofactors. Both intra- and intermolecular recombinations proceed with rapid kinetics. The recombination is highly specific, and no reactions are obser ...[more]