Ontology highlight
ABSTRACT:
SUBMITTER: Singh S
PROVIDER: S-EPMC3642061 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Singh Shweta S Ghosh Pallavi P Hatfull Graham F GF
PLoS genetics 20130502 5
Phage-encoded serine integrases mediate directionally regulated site-specific recombination between short attP and attB DNA sites without host factor requirements. These features make them attractive for genome engineering and synthetic genetics, although the basis for DNA site selection is poorly understood. Here we show that attP selection is determined through multiple proofreading steps that reject non-attP substrates, and that discrimination of attP and attB involves two critical site featu ...[more]