Ontology highlight
ABSTRACT:
SUBMITTER: Mathew-Fenn RS
PROVIDER: S-EPMC2566812 | biostudies-literature | 2008
REPOSITORIES: biostudies-literature
Mathew-Fenn Rebecca S RS Das Rhiju R Silverman Joshua A JA Walker Peter A PA Harbury Pehr A B PA
PloS one 20081017 10
We report a novel molecular ruler for measurement of distances and distance distributions with accurate external calibration. Using solution X-ray scattering we determine the scattering interference between two gold nanocrystal probes attached site-specifically to a macromolecule of interest. Fourier transformation of the interference pattern provides a model-independent probability distribution for the distances between the probe centers-of-mass. To test the approach, we measure end-to-end dist ...[more]