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The nucleosome-remodeling ATPase ISWI is regulated by poly-ADP-ribosylation.

ABSTRACT: ATP-dependent nucleosome-remodeling enzymes and covalent modifiers of chromatin set the functional state of chromatin. However, how these enzymatic activities are coordinated in the nucleus is largely unknown. We found that the evolutionary conserved nucleosome-remodeling ATPase ISWI and the poly-ADP-ribose polymerase PARP genetically interact. We present evidence showing that ISWI is target of poly-ADP-ribosylation. Poly-ADP-ribosylation counteracts ISWI function in vitro and in vivo. Our work suggests that ISWI is a physiological target of PARP and that poly-ADP-ribosylation can be a new, important post-translational modification regulating the activity of ATP-dependent nucleosome remodelers.

SUBMITTER: Sala A

PROVIDER: S-EPMC2567001 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature

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The nucleosome-remodeling ATPase ISWI is regulated by poly-ADP-ribosylation.

Sala Anna A La Rocca Gaspare G Burgio Giosalba G Kotova Elena E Di Gesù Dario D Collesano Marianna M Ingrassia Antonia M R AM Tulin Alexei V AV Corona Davide F V DF

PLoS biology 20081001 10

ATP-dependent nucleosome-remodeling enzymes and covalent modifiers of chromatin set the functional state of chromatin. However, how these enzymatic activities are coordinated in the nucleus is largely unknown. We found that the evolutionary conserved nucleosome-remodeling ATPase ISWI and the poly-ADP-ribose polymerase PARP genetically interact. We present evidence showing that ISWI is target of poly-ADP-ribosylation. Poly-ADP-ribosylation counteracts ISWI function in vitro and in vivo. Our work ...[more]

PMID: 18922045

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