Ontology highlight
ABSTRACT:
SUBMITTER: Yamada M
PROVIDER: S-EPMC2567412 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
Yamada Masami M Toba Shiori S Yoshida Yuko Y Haratani Koji K Mori Daisuke D Yano Yoshihisa Y Mimori-Kiyosue Yuko Y Nakamura Takeshi T Itoh Kyoko K Fushiki Shinji S Setou Mitsutoshi M Wynshaw-Boris Anthony A Torisawa Takayuki T Toyoshima Yoko Y YY Hirotsune Shinji S
The EMBO journal 20080911 19
LIS1 was first identified as a gene mutated in human classical lissencephaly sequence. LIS1 is required for dynein activity, but the underlying mechanism is poorly understood. Here, we demonstrate that LIS1 suppresses the motility of cytoplasmic dynein on microtubules (MTs), whereas NDEL1 releases the blocking effect of LIS1 on cytoplasmic dynein. We demonstrate that LIS1, cytoplasmic dynein and MT fragments co-migrate anterogradely. When LIS1 function was suppressed by a blocking antibody, ante ...[more]