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A novel role for jun N-terminal kinase signaling in olfactory sensory neuronal death.

ABSTRACT: Olfactory sensory neurons (OSNs) represent a unique population of neurons in which death and regeneration are ongoing throughout adulthood, a feature that makes them an attractive model cell type for the investigation of neuronal death. However, the mechanism by which OSNs die remains elusive. Therefore, we developed a culture system for studying pathways involved in OSN death. Here, we show that inhibition of transcription or translation, by actinomycin D or cycloheximide, respectively, suppresses pathways leading to death, prolonging the survival of OSNs in culture. We discovered that caspase activity and jun N-terminal kinase (JNK) signaling both play a role in OSN death, and inhibition of JNK activity suppresses effector caspase (caspase-3) activation. Results from studies in culture were confirmed in vivo, in a mouse bulbectomy-induced OSN death model. These findings provide new insights into the nature of OSN death and a means of studying OSNs in vitro.

SUBMITTER: Gangadhar NM

PROVIDER: S-EPMC2568995 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature

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A novel role for jun N-terminal kinase signaling in olfactory sensory neuronal death.

Gangadhar Nidhi M NM Firestein Stuart J SJ Stockwell Brent R BR

Molecular and cellular neurosciences 20080511 4

Olfactory sensory neurons (OSNs) represent a unique population of neurons in which death and regeneration are ongoing throughout adulthood, a feature that makes them an attractive model cell type for the investigation of neuronal death. However, the mechanism by which OSNs die remains elusive. Therefore, we developed a culture system for studying pathways involved in OSN death. Here, we show that inhibition of transcription or translation, by actinomycin D or cycloheximide, respectively, suppres ...[more]

PMID: 18571430

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