Ontology highlight
ABSTRACT:
SUBMITTER: O'Connell Motherway M
PROVIDER: S-EPMC2570300 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
O'Connell Motherway Mary M Fitzgerald Gerald F GF Neirynck Sabine S Ryan Sinead S Steidler Lothar L van Sinderen Douwe D
Applied and environmental microbiology 20080808 20
The apuB gene of Bifidobacterium breve UCC2003 was shown to encode an extracellular amylopullulanase. ApuB is composed of a distinct N-terminally located alpha-amylase-containing domain which hydrolyzes alpha-1,4-glucosidic linkages in starch and related polysaccharides and a C-terminally located pullulanase-containing domain which hydrolyzes alpha-1,6 linkages in pullulan, allowing the classification of this enzyme as a bifunctional class II pullulanase. A knockout mutation of the apuB gene in ...[more]