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ABSTRACT:
SUBMITTER: Pokusaeva K
PROVIDER: S-EPMC2643558 | biostudies-literature | 2009 Feb
REPOSITORIES: biostudies-literature
Pokusaeva Karina K O'Connell-Motherway Mary M Zomer Aldert A Fitzgerald Gerald F GF van Sinderen Douwe D
Applied and environmental microbiology 20081229 4
Two alpha-glucosidase-encoding genes (agl1 and agl2) from Bifidobacterium breve UCC2003 were identified and characterized. Based on their similarity to characterized carbohydrate hydrolases, the Agl1 and Agl2 enzymes are both assigned to a subgroup of the glycosyl hydrolase family 13, the alpha-1,6-glucosidases (EC 3.2.1.10). Recombinant Agl1 and Agl2 into which a His(12) sequence was incorporated (Agl1(His) and Agl2(His), respectively) exhibited hydrolytic activity towards panose, isomaltose, i ...[more]