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KHM-1, a novel plasmid-mediated metallo-beta-lactamase from a Citrobacter freundii clinical isolate.

ABSTRACT: A novel gene, bla(KHM-1), encoding a metallo-beta-lactamase, KHM-1, was cloned from a clinical isolate of Citrobacter freundii resistant to most beta-lactam antibiotics. Escherichia coli expressing bla(KHM-1) was resistant to all broad-spectrum beta-lactams except for monobactams and showed reduced susceptibility to carbapenems. Recombinant KHM-1 exhibited EDTA-inhibitable hydrolytic activity against most beta-lactams, with an overall preference for cephalosporins.

SUBMITTER: Sekiguchi J

PROVIDER: S-EPMC2573105 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature

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KHM-1, a novel plasmid-mediated metallo-beta-lactamase from a Citrobacter freundii clinical isolate.

Sekiguchi Jun-ichiro J Morita Koji K Kitao Tomoe T Watanabe Noboru N Okazaki Mitsuhiro M Miyoshi-Akiyama Tohru T Kanamori Masato M Kirikae Teruo T

Antimicrobial agents and chemotherapy 20080902 11

A novel gene, bla(KHM-1), encoding a metallo-beta-lactamase, KHM-1, was cloned from a clinical isolate of Citrobacter freundii resistant to most beta-lactam antibiotics. Escherichia coli expressing bla(KHM-1) was resistant to all broad-spectrum beta-lactams except for monobactams and showed reduced susceptibility to carbapenems. Recombinant KHM-1 exhibited EDTA-inhibitable hydrolytic activity against most beta-lactams, with an overall preference for cephalosporins. ...[more]

PMID: 18765691

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