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Identification of Ugandan HIV type 1 variants with unique patterns of recombination in pol involving subtypes A and D.

ABSTRACT: Most HIV-1 infections in Uganda are caused by subtypes A and D. The prevalence of recombination and the sites of specific breakpoints between these subtypes have not been reported. HIV-1 pol sequences encoding protease (amino acids 1-99) and reverse transcriptase (amino acids 1-324) from 102 pregnant Ugandan women were analyzed by the Recombinant Identification Program, SimPlot, and examination of phylogenetically informative sites to identify sites of recombination between sequence segments belonging to different subtypes. Thirteen percent (13 of 102) of the pol sequences contained strong evidence of recombination between subtypes A and D. At least nine different patterns of recombination were observed. Five women infected with a recombinant virus transmitted the recombinant virus perinatally. In this population-based study, intersubtype recombinants were common. The large number of different types of pol recombinants identified suggests that recombination occurs readily in the pol region. Perinatal transmission of the recombinant viruses demonstrates their evolutionary stability.

SUBMITTER: Eshleman SH

PROVIDER: S-EPMC2573392 | biostudies-literature | 2002 May

REPOSITORIES: biostudies-literature

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Identification of Ugandan HIV type 1 variants with unique patterns of recombination in pol involving subtypes A and D.

Eshleman Susan H SH Gonzales Matthew J MJ Becker-Pergola Graziella G Cunningham Shawn C SC Guay Laura A LA Jackson J Brooks JB Shafer Robert W RW

AIDS research and human retroviruses 20020501 7

Most HIV-1 infections in Uganda are caused by subtypes A and D. The prevalence of recombination and the sites of specific breakpoints between these subtypes have not been reported. HIV-1 pol sequences encoding protease (amino acids 1-99) and reverse transcriptase (amino acids 1-324) from 102 pregnant Ugandan women were analyzed by the Recombinant Identification Program, SimPlot, and examination of phylogenetically informative sites to identify sites of recombination between sequence segments bel ...[more]

PMID: 12015904

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Identification of Ugandan HIV type 1 variants with unique patterns of recombination in pol involving subtypes A and D.

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Identification of Ugandan HIV type 1 variants with unique patterns of recombination in pol involving subtypes A and D.

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