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NMR evidence for forming highly populated helical conformations in the partially folded hNck2 SH3 domain.


ABSTRACT: Recent studies of several proteins implied that the folding of beta-proteins may follow a nonhierarchical mechanism in which two major transitions are essential, i.e., the collapse of a random coil to form a nonnative helical intermediate, followed by a transformation into the native beta-structure. We report that the first hNck2 SH3 domain, assuming an all-beta barrel in the native form, can be reversibly transformed into a stable and nonnative helical state by acid-unfolding. We also conducted extensive NMR and mutagenesis studies that led to two striking findings: 1), NMR analysis reveals that in the helical state formed at pH 2.0, the first and last beta-strands in the native form become unstructured, whereas the rest is surprisingly converted into two highly populated helices with a significantly limited backbone motion; and 2), a conserved four-residue sequence is identified on the second beta-strand, a mutation of which suddenly renders the SH3 domain into a helical state even at pH 6.5, with NMR conformational and dynamic properties highly similar to those of the wild-type at pH 2.0. This observation implies that the region might contribute key interactions to disrupt the helical state, and to facilitate a further transformation into the native SH3 fold in the second transition.

SUBMITTER: Liu J 

PROVIDER: S-EPMC2576364 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature

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NMR evidence for forming highly populated helical conformations in the partially folded hNck2 SH3 domain.

Liu Jingxian J   Song Jianxing J  

Biophysical journal 20080703 10


Recent studies of several proteins implied that the folding of beta-proteins may follow a nonhierarchical mechanism in which two major transitions are essential, i.e., the collapse of a random coil to form a nonnative helical intermediate, followed by a transformation into the native beta-structure. We report that the first hNck2 SH3 domain, assuming an all-beta barrel in the native form, can be reversibly transformed into a stable and nonnative helical state by acid-unfolding. We also conducted  ...[more]

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