Ontology highlight
ABSTRACT:
SUBMITTER: Fletcher JI
PROVIDER: S-EPMC2577705 | biostudies-literature | 2008 Nov
REPOSITORIES: biostudies-literature
Fletcher Jamie I JI Meusburger Sarina S Hawkins Christine J CJ Riglar David T DT Lee Erinna F EF Fairlie W Douglas WD Huang David C S DC Adams Jerry M JM
Proceedings of the National Academy of Sciences of the United States of America 20081103 47
A central issue in the control of apoptosis is whether its essential mediators Bax and Bak must be restrained by Bcl-2-like prosurvival relatives to prevent their damaging mitochondria and unleashing apoptosis. The issue is particularly vexed for Bax, which is largely a cytosolic monomer in unstressed cells. To determine whether Bax regulation requires its binding by prosurvival relatives, we replaced a conserved aspartate in its BH3 interaction domain with arginine. Bax D68R functioned and beha ...[more]