Ontology highlight
ABSTRACT:
SUBMITTER: Bockhorn J
PROVIDER: S-EPMC2584752 | biostudies-literature | 2008 Nov
REPOSITORIES: biostudies-literature
Bockhorn Jessica J Balar Bharvi B He Dongming D Seitomer Eden E Copeland Paul R PR Kinzy Terri Goss TG
Proceedings of the National Academy of Sciences of the United States of America 20081111 46
Selenomethionine (SeMet) is a potentially toxic amino acid, and yet it is a valuable tool in the preparation of labeled proteins for multiwavelength anomalous dispersion or single-wavelength anomalous dispersion phasing in X-ray crystallography. The mechanism by which high levels of SeMet exhibits its toxic effects in eukaryotic cells is not fully understood. Attempts to use Saccharomyces cerevisiae for the preparation of fully substituted SeMet proteins for X-ray crystallography have been limit ...[more]