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Estimating kinetic and thermodynamic parameters from single molecule enzyme-inhibitor interactions.

ABSTRACT: We report the application of recently developed microscopic models to estimate the apparent kinetic and thermodynamic parameters in a single molecule force spectroscopy study of the carbonic anhydrase enzyme and a complementary sulfonamide inhibitor. The most probable rupture force for the enzyme-inhibitor interaction shows a nonlinear dependency on the log-loading rate. Estimates for the kinetic and thermodynamic parameters were obtained by fitting the nonlinear dependency to linear cubic potential and cusp potential models and compared to the standard Bell-Evans model. The reliability of the estimated parameters was verified by modeling the experimental rupture force distributions by the theoretically predicted distributions at rupture. We also report that linkers that are attached to the enzyme and inhibitor show appreciable effects on the apparent kinetic and thermodynamic parameters.

SUBMITTER: Porter-Peden L

PROVIDER: S-EPMC2586077 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature

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Estimating kinetic and thermodynamic parameters from single molecule enzyme-inhibitor interactions.

Porter-Peden Laura L Kamper Sarah G SG Wal Mark Vander MV Blankespoor Ronald R Sinniah Kumar K

Langmuir : the ACS journal of surfaces and colloids 20080923 20

We report the application of recently developed microscopic models to estimate the apparent kinetic and thermodynamic parameters in a single molecule force spectroscopy study of the carbonic anhydrase enzyme and a complementary sulfonamide inhibitor. The most probable rupture force for the enzyme-inhibitor interaction shows a nonlinear dependency on the log-loading rate. Estimates for the kinetic and thermodynamic parameters were obtained by fitting the nonlinear dependency to linear cubic poten ...[more]

PMID: 18808161

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Dataset Information

Estimating kinetic and thermodynamic parameters from single molecule enzyme-inhibitor interactions.

Publications

Estimating kinetic and thermodynamic parameters from single molecule enzyme-inhibitor interactions.

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