Ontology highlight
ABSTRACT:
SUBMITTER: Ponzielli R
PROVIDER: S-EPMC2588500 | biostudies-literature | 2008 Dec
REPOSITORIES: biostudies-literature
Ponzielli Romina R Boutros Paul C PC Katz Sigal S Stojanova Angelina A Hanley Adam P AP Khosravi Fereshteh F Bros Christina C Jurisica Igor I Penn Linda Z LZ
Nucleic acids research 20081021 21
High-throughput, microarray-based chromatin immunoprecipitation (ChIP-chip) technology allows in vivo elucidation of transcriptional networks. However this complex is not yet readily accessible, in part because its many parameters have not been systematically evaluated and optimized. We address this gap by systematically assessing experimental-design parameters including antibody purity, dye-bias, array-batch, inter-day hybridization bias, amplification method and choice of hybridization control ...[more]