Ontology highlight
ABSTRACT:
SUBMITTER: Chabbert CD
PROVIDER: S-EPMC4332152 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
Chabbert Christophe D CD Adjalley Sophie H SH Klaus Bernd B Fritsch Emilie S ES Gupta Ishaan I Pelechano Vicent V Steinmetz Lars M LM
Molecular systems biology 20150112 1
We present a modified approach of chromatin immuno-precipitation followed by sequencing (ChIP-Seq), which relies on the direct ligation of molecular barcodes to chromatin fragments, thereby permitting experimental scale-up. With Bar-ChIP now enabling the concurrent profiling of multiple DNA-protein interactions, we report the simultaneous generation of 90 ChIP-Seq datasets without any robotic instrumentation. We demonstrate that application of Bar-ChIP to a panel of Saccharomyces cerevisiae chro ...[more]