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Orally bioavailable potent soluble epoxide hydrolase inhibitors.

ABSTRACT: A series of N,N'-disubstituted ureas having a conformationally restricted cis- or trans-1,4-cyclohexane alpha to the urea were prepared and tested as soluble epoxide hydrolase (sEH) inhibitors. This series of compounds showed low nanomolar to picomolar activities against recombinant human sEH. Both isomers showed similar potencies, but the trans isomers were more metabolically stable in human hepatic microsomes. Furthermore, these new potent inhibitors show a greater metabolic stability in vivo than previously described sEH inhibitors. We demonstrated that trans-4-[4-(3-adamantan-1-ylureido)cyclohexyloxy]benzoic acid 13g (t-AUCB, IC50 = 1.3 +/- 0.05 nM) had excellent oral bioavailability (98%, n = 2) and blood area under the curve in dogs and was effective in vivo to treat hypotension in lipopolysaccharide challenged murine models.

SUBMITTER: Hwang SH

PROVIDER: S-EPMC2596069 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature

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Orally bioavailable potent soluble epoxide hydrolase inhibitors.

Hwang Sung Hee SH Tsai Hsing-Ju HJ Liu Jun-Yan JY Morisseau Christophe C Hammock Bruce D BD

Journal of medicinal chemistry 20070706 16

A series of N,N'-disubstituted ureas having a conformationally restricted cis- or trans-1,4-cyclohexane alpha to the urea were prepared and tested as soluble epoxide hydrolase (sEH) inhibitors. This series of compounds showed low nanomolar to picomolar activities against recombinant human sEH. Both isomers showed similar potencies, but the trans isomers were more metabolically stable in human hepatic microsomes. Furthermore, these new potent inhibitors show a greater metabolic stability in vivo ...[more]

PMID: 17616115

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