Ontology highlight
ABSTRACT:
SUBMITTER: Paatero AO
PROVIDER: S-EPMC2602771 | biostudies-literature | 2008 Dec
REPOSITORIES: biostudies-literature
Paatero Anja O AO Turakainen Hilkka H Happonen Lotta J LJ Olsson Cia C Palomäki Tiina T Pajunen Maria I MI Meng Xiaojuan X Otonkoski Timo T Tuuri Timo T Berry Charles C Malani Nirav N Frilander Mikko J MJ Bushman Frederic D FD Savilahti Harri H
Nucleic acids research 20081025 22
Genomic parasites have evolved distinctive lifestyles to optimize replication in the context of the genomes they inhabit. Here, we introduced new DNA into eukaryotic cells using bacteriophage Mu DNA transposition complexes, termed 'transpososomes'. Following electroporation of transpososomes and selection for marker gene expression, efficient integration was verified in yeast, mouse and human genomes. Although Mu has evolved in prokaryotes, strong biases were seen in the target site distribution ...[more]