Ontology highlight
ABSTRACT:
SUBMITTER: Li H
PROVIDER: S-EPMC2603134 | biostudies-literature | 2008 Nov
REPOSITORIES: biostudies-literature
Li Hong H Miller Paul S PS Seidman Michael M MM
Organic & biomolecular chemistry 20080923 22
Triplex forming oligonucleotides (TFOs) containing the nucleoside analogues 2'-O-methyl-5-propynyluridine (1) and 2'-O-methyl-5-(3-amino-1-propynyl)uridine (2) were synthesized. The affinity and selectivity of triplex formation by these TFOs were studied by gel shift analysis, T(m) value measurement, and association rate assays. The results show that the introduction of 1 and 2 into TFOs can improve the stability of the triplexes under physiological conditions. Optimized distribution of 1 or 2 i ...[more]