Ontology highlight
ABSTRACT:
SUBMITTER: Guo S
PROVIDER: S-EPMC2613637 | biostudies-literature | 2009 Jan
REPOSITORIES: biostudies-literature
Guo Shuangli S Burnette Ryan R Zhao Li L Vanderford Nathan L NL Poitout Vincent V Hagman Derek K DK Henderson Eva E Ozcan Sabire S Wadzinski Brian E BE Stein Roland R
The Journal of biological chemistry 20081112 2
The level of the MafA transcription factor is regulated by a variety of effectors of beta cell function, including glucose, fatty acids, and insulin. Here, we show that phosphorylation at Ser(65) of mammalian MafA influences both protein stability and transactivation potential. Replacement of Ser(65) with Glu to mimic phosphorylation produced a protein that was as unstable as the wild type, whereas Asp or Ala mutation blocked degradation. Analysis of MafA chimeric and deletion constructs suggest ...[more]