Ontology highlight
ABSTRACT:
SUBMITTER: Cho BK
PROVIDER: S-EPMC2614783 | biostudies-literature | 2008 Dec
REPOSITORIES: biostudies-literature
Cho Byung-Kwan BK Barrett Christian L CL Knight Eric M EM Park Young Seoub YS Palsson Bernhard Ø BØ
Proceedings of the National Academy of Sciences of the United States of America 20081203 49
Broad-acting transcription factors (TFs) in bacteria form regulons. Here, we present a 4-step method to fully reconstruct the leucine-responsive protein (Lrp) regulon in Escherichia coli K-12 MG 1655 that regulates nitrogen metabolism. Step 1 is composed of obtaining high-resolution ChIP-chip data for Lrp, the RNA polymerase and expression profiles under multiple environmental conditions. We identified 138 unique and reproducible Lrp-binding regions and classified their binding state under diffe ...[more]