Characterization of denitrification gene clusters of soil bacteria via a metagenomic approach.
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ABSTRACT:
SUBMITTER: Demaneche S
PROVIDER: S-EPMC2620714 | biostudies-literature | 2009 Jan
REPOSITORIES: biostudies-literature
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Characterization of denitrification gene clusters of soil bacteria via a metagenomic approach.
Demanèche Sandrine S Philippot Laurent L David Maude M MM Navarro Elisabeth E Vogel Timothy M TM Simonet Pascal P
Applied and environmental microbiology 20081114 2
We characterized operons encoding enzymes involved in denitrification, a nitrogen-cycling process involved in nitrogen losses and greenhouse gas emission, using a metagenomic approach which combines molecular screening and pyrosequencing. Screening of 77,000 clones from a soil metagenomic library led to the identification and the subsequent characterization of nine denitrification gene clusters. ...[more]
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