Ontology highlight
ABSTRACT:
SUBMITTER: Lopez I
PROVIDER: S-EPMC262258 | biostudies-literature | 2003 Nov
REPOSITORIES: biostudies-literature
Lopez Isabel I Ruiz-Larrea Fernanda F Cocolin Luca L Orr Erica E Phister Trevor T Marshall Megan M VanderGheynst Jean J Mills David A DA
Applied and environmental microbiology 20031101 11
Denaturing gradient gel electrophoresis (DGGE) of PCR-amplified ribosomal DNA (rDNA) is routinely used to compare levels of diversity of microbial communities and to monitor population dynamics. While using PCR-DGGE to examine the bacteria in wine fermentations, we noted that several commonly used PCR primers for amplifying bacterial 16S rDNA also coamplified yeast, fungal, or plant DNA present in samples. Unfortunately, amplification of nonbacterial DNA can result in a masking of bacterial popu ...[more]